Role of Na+/H+ exchanger isoform-1 in human inflammatory bowel disease
Cations such as sodium (Na+), potassium and calcium (Ca2+) play an important role in several key biological processes. Homeostasis of these ions is compromised in inflammatory bowel disease (IBD),...
View ArticleRole of Na+/H+ exchanger isoform-1 in human inflammatory bowel disease:...
Collection of human biopsies Twenty-seven patients with chronic diarrhea and undergoing initial colonoscopy for suspected IBD were invited to participate in the study from February 1 to June 30, 2002....
View ArticleRole of Na+/H+ exchanger isoform-1 in human inflammatory bowel disease:...
Sodium pump activity Potassium-stimulated-ouabain sensitive p-nitrophenylphos-phatase is a partial activity of and is used as a marker of the sodium pump. Aliquots (10 to 20 ug) of crude microsomes...
View ArticleRole of Na+/H+ exchanger isoform-1 in human inflammatory bowel disease:...
Subjects This study used a total of 35 patients that included CD (n= 13), UC (n=11) and normal control (n=11) subjects. In each group, both male and female subjects were recruited whose mean age in...
View ArticleRole of Na+/H+ exchanger isoform-1 in human inflammatory bowel disease:...
NHE-1 protein and mRNA Crude microsomal yield from the CD and UC patients’ biopsies was not significantly (P>0.05) different from the normal control (Figure 3). However, the yield of crude...
View ArticleRole of Na+/H+ exchanger isoform-1 in human inflammatory bowel disease:...
NHE-1 mRNA level were also decreased significantly both in the UC and CD patients’ biopsies (Figure 6), whereas the internal control level of actin mRNA remained unchanged in these groups (Figure 6)....
View ArticleRole of Na+/H+ exchanger isoform-1 in human inflammatory bowel disease:...
The basis of this study came from recent findings reporting induction of NHE-1 and -3 isoforms in the colonileum and kidneys in experimental colitis. In addition, a recent study has provided evidence...
View ArticleRole of Na+/H+ exchanger isoform-1 in human inflammatory bowel disease:...
Based on animal model studies, we expected an induction of NHE-1 expression in human IBD, but it was suppressed in both CD and UC patients, indicating a pathophysiological disparity between the animal...
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